Selective Release of Recombinant Periplasmic Protein From E. coli Using Continuous Pulsed Electric Field Treatment

Selective Release of Recombinant Periplasmic Protein From E. coli Using Continuous Pulsed Electric Field Treatment

To date, high-pressure homogenization is the standard method for cell disintegration before the extraction of cytosolic and periplasmic protein from E. coli. Its main drawback, however, is low selectivity and a resulting high load of host cell impurities. Pulsed electric field (PEF) treatment may be...

Full description

Bibliographic Details
Main Authors: Felix Schottroff, Jens Kastenhofer, Oliver Spadiut, Henry Jaeger, David J. Wurm
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-02-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
continuous downstream processing
electroporation
host cell impurities
outer membrane
periplasmic protein
primary recovery
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2020.586833/full

Internet

https://www.frontiersin.org/articles/10.3389/fbioe.2020.586833/full

Similar Items