Analysis of proteostasis during aging with western blot of detergent-soluble and insoluble protein fractions

Analysis of proteostasis during aging with western blot of detergent-soluble and insoluble protein fractions

Summary: Defects in protein quality control are the underlying cause of age-related diseases. The western blot analysis of detergent-soluble and insoluble protein fractions has proven useful in identifying interventions that regulate proteostasis. Here, we describe the protocol for such analyses in...

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Bibliographic Details
Main Authors: Mamta Rai, Michelle Curley, Zane Coleman, Anjana Nityanandam, Jianqin Jiao, Flavia A. Graca, Liam C. Hunt, Fabio Demontis
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Cell Biology
Developmental biology
Model Organisms
Molecular Biology
Protein Biochemistry
Organoids
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672100335X

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http://www.sciencedirect.com/science/article/pii/S266616672100335X

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