Glycine Regulates Neural Stem Cell Proliferation During Development via Lnx1-Dependent Notch Signaling
During development of the zebrafish embryo, glycine signaling promotes the differentiation of neural stem cells (NSCs). We found that glycine signaling suppresses the expression of Ligand of Numb X1 (lnx1, Ligand of numb protein-x1), a gene of unknown function during NSC differentiation that is sele...
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doaj-ca686e5eeee04c528cfed84030ce8aad2020-11-24T23:56:45ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992019-02-011210.3389/fnmol.2019.00044438576Glycine Regulates Neural Stem Cell Proliferation During Development via Lnx1-Dependent Notch SignalingAbdelhamid Bekri0Abdelhamid Bekri1Meijiang Liao2Pierre Drapeau3Pierre Drapeau4Research Center of the University of Montreal Hospital Center (CRCHUM), University of Montreal, Montreal, QC, CanadaDepartment of Biochemistry, University of Montreal, Montreal, QC, CanadaResearch Center of the University of Montreal Hospital Center (CRCHUM), University of Montreal, Montreal, QC, CanadaResearch Center of the University of Montreal Hospital Center (CRCHUM), University of Montreal, Montreal, QC, CanadaDepartment of Neuroscience, University of Montreal, Montreal, QC, CanadaDuring development of the zebrafish embryo, glycine signaling promotes the differentiation of neural stem cells (NSCs). We found that glycine signaling suppresses the expression of Ligand of Numb X1 (lnx1, Ligand of numb protein-x1), a gene of unknown function during NSC differentiation that is selectively expressed in the embryonic central nervous system (CNS). As a consequence, Numb levels were stabilized and Notch activity (measured as her4.1 expression) was reduced, promoting NSC differentiation. These consequent actions were blocked by knockdown of lnx1. In contrast, lnx1 overexpression increased NSC proliferation and led to defects of neural tube closure at the early stages of development. Thus, our data provide evidence that glycine/lnx1 signaling modulates NSC proliferation by regulation of Notch signaling.https://www.frontiersin.org/article/10.3389/fnmol.2019.00044/fullLNX1NSCsglycine signalingneurogenesisNotch activity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Abdelhamid Bekri Abdelhamid Bekri Meijiang Liao Pierre Drapeau Pierre Drapeau |
spellingShingle |
Abdelhamid Bekri Abdelhamid Bekri Meijiang Liao Pierre Drapeau Pierre Drapeau Glycine Regulates Neural Stem Cell Proliferation During Development via Lnx1-Dependent Notch Signaling Frontiers in Molecular Neuroscience LNX1 NSCs glycine signaling neurogenesis Notch activity |
author_facet |
Abdelhamid Bekri Abdelhamid Bekri Meijiang Liao Pierre Drapeau Pierre Drapeau |
author_sort |
Abdelhamid Bekri |
title |
Glycine Regulates Neural Stem Cell Proliferation During Development via Lnx1-Dependent Notch Signaling |
title_short |
Glycine Regulates Neural Stem Cell Proliferation During Development via Lnx1-Dependent Notch Signaling |
title_full |
Glycine Regulates Neural Stem Cell Proliferation During Development via Lnx1-Dependent Notch Signaling |
title_fullStr |
Glycine Regulates Neural Stem Cell Proliferation During Development via Lnx1-Dependent Notch Signaling |
title_full_unstemmed |
Glycine Regulates Neural Stem Cell Proliferation During Development via Lnx1-Dependent Notch Signaling |
title_sort |
glycine regulates neural stem cell proliferation during development via lnx1-dependent notch signaling |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Molecular Neuroscience |
issn |
1662-5099 |
publishDate |
2019-02-01 |
description |
During development of the zebrafish embryo, glycine signaling promotes the differentiation of neural stem cells (NSCs). We found that glycine signaling suppresses the expression of Ligand of Numb X1 (lnx1, Ligand of numb protein-x1), a gene of unknown function during NSC differentiation that is selectively expressed in the embryonic central nervous system (CNS). As a consequence, Numb levels were stabilized and Notch activity (measured as her4.1 expression) was reduced, promoting NSC differentiation. These consequent actions were blocked by knockdown of lnx1. In contrast, lnx1 overexpression increased NSC proliferation and led to defects of neural tube closure at the early stages of development. Thus, our data provide evidence that glycine/lnx1 signaling modulates NSC proliferation by regulation of Notch signaling. |
topic |
LNX1 NSCs glycine signaling neurogenesis Notch activity |
url |
https://www.frontiersin.org/article/10.3389/fnmol.2019.00044/full |
work_keys_str_mv |
AT abdelhamidbekri glycineregulatesneuralstemcellproliferationduringdevelopmentvialnx1dependentnotchsignaling AT abdelhamidbekri glycineregulatesneuralstemcellproliferationduringdevelopmentvialnx1dependentnotchsignaling AT meijiangliao glycineregulatesneuralstemcellproliferationduringdevelopmentvialnx1dependentnotchsignaling AT pierredrapeau glycineregulatesneuralstemcellproliferationduringdevelopmentvialnx1dependentnotchsignaling AT pierredrapeau glycineregulatesneuralstemcellproliferationduringdevelopmentvialnx1dependentnotchsignaling |
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