Stereoselectivity Switch in the Reduction of α-Alkyl-β-Arylenones by Structure-Guided Designed Variants of the Ene Reductase OYE1

Stereoselectivity Switch in the Reduction of α-Alkyl-β-Arylenones by Structure-Guided Designed Variants of the Ene Reductase OYE1

Ene reductases from the Old Yellow Enzyme (OYE) family are industrially interesting enzymes for the biocatalytic asymmetric reduction of alkenes. To access both enantiomers of the target reduced products, stereocomplementary pairs of OYE enzymes are necessary, but their natural occurrence is quite l...

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Bibliographic Details
Main Authors: Michele Crotti, Fabio Parmeggiani, Erica Elisa Ferrandi, Francesco G. Gatti, Alessandro Sacchetti, Sergio Riva, Elisabetta Brenna, Daniela Monti
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-04-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
ene reductases
old yellow enzymes
protein engineering
biocatalysis
stereoselectivity
reduction
Online Access:https://www.frontiersin.org/article/10.3389/fbioe.2019.00089/full

Internet

https://www.frontiersin.org/article/10.3389/fbioe.2019.00089/full

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