A rapid in vitro method to flip back the double-floxed inverted open reading frame in a plasmid
Abstract Background The LoxP site based genetic switch, the FLEX, also known as DIO (Double-Floxed Inverted Open reading frame), was invented to turn on gene expression via Cre-mediated recombination. Since its first publication, numerous FLEX switch plasmids have been generated. These plasmids are...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-08-01
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| Series: | BMC Biotechnology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12896-018-0462-x |