Backbone-independent NMR resonance assignments of methyl probes in large proteins

Here, the authors present Methyl Assignments Using Satisfiability (MAUS), a method for the assignment of methyl groups using raw NOE data. They use eight proteins in the 10–45 kDa size range as test cases and show that MAUS yields 100% accurate assignments at high completeness levels.

Bibliographic Details
Main Authors: Santrupti Nerli, Viviane S. De Paula, Andrew C. McShan, Nikolaos G. Sgourakis
Format: Article
Language:English
Published: Nature Publishing Group 2021-01-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-021-20984-0