Three-Dimensional Observations of an Aperiodic Oscillatory Gliding Behavior in Myxococcus xanthus Using Confocal Interference Reflection Microscopy

Three-Dimensional Observations of an Aperiodic Oscillatory Gliding Behavior in Myxococcus xanthus Using Confocal Interference Reflection Microscopy

3D imaging of live bacteria with optical microscopy techniques is a challenge due to the small size of bacterial cells, meaning that previous studies have been limited to observing motility behavior in 2D. We introduce the application of confocal multiwavelength interference reflection microscopy to...

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Bibliographic Details
Main Authors: Liam M. Rooney, Lisa S. Kölln, Ross Scrimgeour, William B. Amos, Paul A. Hoskisson, Gail McConnell
Format: Article
Language:English
Published: American Society for Microbiology 2020-01-01
Series:mSphere
Subjects:
3d imaging
bacterial motility
gliding motility
label free
live-cell imaging
optical microscopy
Online Access:https://doi.org/10.1128/mSphere.00846-19

Internet

https://doi.org/10.1128/mSphere.00846-19

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