A method for conducting suppression assays using small numbers of tissue-isolated regulatory T cells

The suppression assay is a commonly performed assay, measuring the ability of regulatory T cells (Treg) to suppress T cell proliferation. Most frequently, Treg are obtained from the peripheral blood or spleen. Lower yields are obtained by isolation from other tissues, rendering downstream suppressio...

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Bibliographic Details
Main Authors: Stephen T. Ward, Ka-Kit Li, Stuart M. Curbishley
Format: Article
Language:English
Published: Elsevier 2014-01-01
Series:MethodsX
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016114000107