Development of an NGS-Based Workflow for Improved Monitoring of Circulating Plasmids in Support of Risk Assessment of Antimicrobial Resistance Gene Dissemination

Development of an NGS-Based Workflow for Improved Monitoring of Circulating Plasmids in Support of Risk Assessment of Antimicrobial Resistance Gene Dissemination

Antimicrobial resistance (AMR) is one of the most prominent public health threats. AMR genes localized on plasmids can be easily transferred between bacterial isolates by horizontal gene transfer, thereby contributing to the spread of AMR. Next-generation sequencing (NGS) technologies are ideal for...

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Bibliographic Details
Main Authors: Bas Berbers, Pieter-Jan Ceyssens, Pierre Bogaerts, Kevin Vanneste, Nancy H. C. Roosens, Kathleen Marchal, Sigrid C. J. De Keersmaecker
Format: Article
Language:English
Published: MDPI AG 2020-08-01
Series:Antibiotics
Subjects:
plasmids
antimicrobial resistance
conjugation
DNA extraction
next-generation sequencing
MiSeq
Online Access:https://www.mdpi.com/2079-6382/9/8/503

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https://www.mdpi.com/2079-6382/9/8/503

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