Establishment of induced pluripotent stem cells from centenarians for neurodegenerative disease research.
Induced pluripotent stem cell (iPSC) technology can be used to model human disorders, create cell-based models of human diseases, including neurodegenerative diseases, and in establishing therapeutic strategies. To detect subtle cellular abnormalities associated with common late-onset disease in iPS...
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2012-01-01
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doaj-cbeafbcd106045e3becdcd35fdd54c312021-03-04T00:29:54ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0177e4157210.1371/journal.pone.0041572Establishment of induced pluripotent stem cells from centenarians for neurodegenerative disease research.Takuya YagiArifumi KosakaiDaisuke ItoYohei OkadaWado AkamatsuYoshihiro NiheiAkira NabetaniFuyuki IshikawaYasumichi AraiNobuyoshi HiroseHideyuki OkanoNorihiro SuzukiInduced pluripotent stem cell (iPSC) technology can be used to model human disorders, create cell-based models of human diseases, including neurodegenerative diseases, and in establishing therapeutic strategies. To detect subtle cellular abnormalities associated with common late-onset disease in iPSCs, valid control iPSCs derived from healthy donors free of serious late-onset diseases are necessary. Here, we report the generation of iPSCs from fibroblasts obtained immediately postmortem from centenarian donors (106- and 109-years-old) who were extremely healthy until an advanced age. The iPSCs were generated using a conventional method involving OCT4, SOX2, KLF4, and c-MYC, and then differentiated into neuronal cells using a neurosphere method. The expression of molecules that play critical roles in late-onset neurodegenerative diseases by neurons differentiated from the centenarian-iPSCs was compared to that of neurons differentiated from iPSCs derived from familial Alzheimer's disease and familial Parkinson's disease (PARK4: triplication of the α synuclein gene) patients. The results indicated that our series of iPSCs would be useful in neurodegeneration research. The iPSCs we describe, which were derived from donors with exceptional longevity who were presumed to have no serious disease risk factors, would be useful in longevity research and as valid super-controls for use in studies of various late-onset diseases.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22848530/pdf/?tool=EBI |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Takuya Yagi Arifumi Kosakai Daisuke Ito Yohei Okada Wado Akamatsu Yoshihiro Nihei Akira Nabetani Fuyuki Ishikawa Yasumichi Arai Nobuyoshi Hirose Hideyuki Okano Norihiro Suzuki |
spellingShingle |
Takuya Yagi Arifumi Kosakai Daisuke Ito Yohei Okada Wado Akamatsu Yoshihiro Nihei Akira Nabetani Fuyuki Ishikawa Yasumichi Arai Nobuyoshi Hirose Hideyuki Okano Norihiro Suzuki Establishment of induced pluripotent stem cells from centenarians for neurodegenerative disease research. PLoS ONE |
author_facet |
Takuya Yagi Arifumi Kosakai Daisuke Ito Yohei Okada Wado Akamatsu Yoshihiro Nihei Akira Nabetani Fuyuki Ishikawa Yasumichi Arai Nobuyoshi Hirose Hideyuki Okano Norihiro Suzuki |
author_sort |
Takuya Yagi |
title |
Establishment of induced pluripotent stem cells from centenarians for neurodegenerative disease research. |
title_short |
Establishment of induced pluripotent stem cells from centenarians for neurodegenerative disease research. |
title_full |
Establishment of induced pluripotent stem cells from centenarians for neurodegenerative disease research. |
title_fullStr |
Establishment of induced pluripotent stem cells from centenarians for neurodegenerative disease research. |
title_full_unstemmed |
Establishment of induced pluripotent stem cells from centenarians for neurodegenerative disease research. |
title_sort |
establishment of induced pluripotent stem cells from centenarians for neurodegenerative disease research. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
Induced pluripotent stem cell (iPSC) technology can be used to model human disorders, create cell-based models of human diseases, including neurodegenerative diseases, and in establishing therapeutic strategies. To detect subtle cellular abnormalities associated with common late-onset disease in iPSCs, valid control iPSCs derived from healthy donors free of serious late-onset diseases are necessary. Here, we report the generation of iPSCs from fibroblasts obtained immediately postmortem from centenarian donors (106- and 109-years-old) who were extremely healthy until an advanced age. The iPSCs were generated using a conventional method involving OCT4, SOX2, KLF4, and c-MYC, and then differentiated into neuronal cells using a neurosphere method. The expression of molecules that play critical roles in late-onset neurodegenerative diseases by neurons differentiated from the centenarian-iPSCs was compared to that of neurons differentiated from iPSCs derived from familial Alzheimer's disease and familial Parkinson's disease (PARK4: triplication of the α synuclein gene) patients. The results indicated that our series of iPSCs would be useful in neurodegeneration research. The iPSCs we describe, which were derived from donors with exceptional longevity who were presumed to have no serious disease risk factors, would be useful in longevity research and as valid super-controls for use in studies of various late-onset diseases. |
url |
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22848530/pdf/?tool=EBI |
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