A real-time PCR-based quantitative assay for 3-methylcytosine demethylase activity of ALKBH3

A real-time PCR-based quantitative assay for 3-methylcytosine demethylase activity of ALKBH3

Human AlkB homolog 3 (ALKBH3), a homolog of the Escherichia coli protein AlkB, demethylates 1-methyladenine and 3-methylcytosine (3-meC) in single-stranded DNA and RNA by oxidative demethylation. Immunohistochemical analyses on clinical cancer specimens and knockdown experiments using RNA interferen...

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Bibliographic Details
Main Authors: Yuko Ueda, Kaori Kitae, Ikumi Ooshio, Yasuyuki Fusamae, Megumi Kawaguchi, Kentaro Jingushi, Kazuo Harada, Kazumasa Hirata, Kazutake Tsujikawa
Format: Article
Language:English
Published: Elsevier 2016-03-01
Series:Biochemistry and Biophysics Reports
Subjects:
AlkB
ALKBH3
Demethylation
3-methylcytosine
RT-PCR
Online Access:http://www.sciencedirect.com/science/article/pii/S2405580816300139

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http://www.sciencedirect.com/science/article/pii/S2405580816300139

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