An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol

<p>Abstract</p> <p>Background</p> <p>Mutagenesis plays an essential role in molecular biology and biochemistry. It has also been used in enzymology and protein science to generate proteins which are more tractable for biophysical techniques. The ability to quickly and s...

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Bibliographic Details
Main Authors: Naismith James H, Liu Huanting
Format: Article
Language:English
Published: BMC 2008-12-01
Series:BMC Biotechnology
Online Access:http://www.biomedcentral.com/1472-6750/8/91