High-resolution melting genotyping of Enterococcus faecium based on multilocus sequence typing derived single nucleotide polymorphisms.

High-resolution melting genotyping of Enterococcus faecium based on multilocus sequence typing derived single nucleotide polymorphisms.

We have developed a single nucleotide polymorphism (SNP) nucleated high-resolution melting (HRM) technique to genotype Enterococcus faecium. Eight SNPs were derived from the E. faecium multilocus sequence typing (MLST) database and amplified fragments containing these SNPs were interrogated by HRM....

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Bibliographic Details
Main Authors: Steven Y C Tong, Shirley Xie, Leisha J Richardson, Susan A Ballard, Farshid Dakh, Elizabeth A Grabsch, M Lindsay Grayson, Benjamin P Howden, Paul D R Johnson, Philip M Giffard
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3241712?pdf=render

Internet

http://europepmc.org/articles/PMC3241712?pdf=render

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