Creation and validation of a ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells
<p>Abstract</p> <p>Background</p> <p>Cloning vectors capable of retroviral transduction have enabled stable gene overexpression in numerous mitotic cell lines. However, the relatively small number of feasible restriction enzyme sequences in their cloning sites can hinde...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-01-01
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| Series: | BMC Biotechnology |
| Online Access: | http://www.biomedcentral.com/1472-6750/12/3 |