Dual RNA-Sequencing of Mycobacterium tuberculosis-Infected Cells from a Murine Infection Model

Summary: Dual RNA-sequencing is a powerful technique to assess both bacterial and host transcriptomes in an unbiased way. We developed a protocol to perform Dual RNA-seq on in vivo-derived macrophage populations infected with Mycobacterium tuberculosis. Here, we provide a practical step-by-step guid...

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Bibliographic Details
Main Authors: Davide Pisu, Lu Huang, Bom Nae Rin Lee, Jennifer K. Grenier, David G. Russell
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:STAR Protocols
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720301106