Importance of experimental information (metadata) for archived sequence data: case of specific gene bias due to lag time between sample harvest and RNA protection in RNA sequencing

Large volumes of high-throughput sequencing data have been submitted to the Sequencing Read Archive (SRA). The lack of experimental metadata associated with the data makes reuse and understanding data quality very difficult. In the case of RNA sequencing (RNA-Seq), which reveals the presence and qua...

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Bibliographic Details
Main Author: Tomoko Matsuda
Format: Article
Language:English
Published: PeerJ Inc. 2021-08-01
Series:PeerJ
Subjects:
Online Access:https://peerj.com/articles/11875.pdf