Folding and Oligomerization of the gp2b/gp3/gp4 Spike Proteins of Equine Arteritis Virus in Vitro

Equine arteritis virus (EAV) is a small, positive-stranded RNA virus. The glycoproteins gp2b, gp3 and gp4 form a heterotrimer in the viral envelope, which is required for cell entry of EAV. We describe expression of the ectodomains of the proteins in E. coli and their refolding from inclusion bodies...

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Bibliographic Details
Main Authors: Aleksander Kabatek, Michael Veit
Format: Article
Language:English
Published: MDPI AG 2012-03-01
Series:Viruses
Subjects:
gp3
gp4
Online Access:http://www.mdpi.com/1999-4915/4/3/414/