Folding and Oligomerization of the gp2b/gp3/gp4 Spike Proteins of Equine Arteritis Virus in Vitro
Equine arteritis virus (EAV) is a small, positive-stranded RNA virus. The glycoproteins gp2b, gp3 and gp4 form a heterotrimer in the viral envelope, which is required for cell entry of EAV. We describe expression of the ectodomains of the proteins in E. coli and their refolding from inclusion bodies...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2012-03-01
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Series: | Viruses |
Subjects: | |
Online Access: | http://www.mdpi.com/1999-4915/4/3/414/ |