Large-Area Fluorescence and Electron Microscopic Correlative Imaging With Multibeam Scanning Electron Microscopy

Large-Area Fluorescence and Electron Microscopic Correlative Imaging With Multibeam Scanning Electron Microscopy

Recent improvements in correlative light and electron microscopy (CLEM) technology have led to dramatic improvements in the ability to observe tissues and cells. Fluorescence labeling has been used to visualize the localization of molecules of interest through immunostaining or genetic modification...

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Bibliographic Details
Main Authors: Shinsuke Shibata, Taro Iseda, Takayuki Mitsuhashi, Atsushi Oka, Tomoko Shindo, Nobuko Moritoki, Toshihiro Nagai, Shinya Otsubo, Takashi Inoue, Erika Sasaki, Chihiro Akazawa, Takao Takahashi, Richard Schalek, Jeff W. Lichtman, Hideyuki Okano
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-05-01
Series:Frontiers in Neural Circuits
Subjects:
correlative imaging
immuno-EM
CLEM
connectomics
multibeam SEM
Online Access:https://www.frontiersin.org/article/10.3389/fncir.2019.00029/full

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https://www.frontiersin.org/article/10.3389/fncir.2019.00029/full

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