Comparison of Five TRIzol-Based Protein Preparation Methods for 2-DE Production From Challenging Marine Dinoflagellate Samples: A Case Study on Two Benthic <i>Prorocentrum</i> Species

Comparison of Five TRIzol-Based Protein Preparation Methods for 2-DE Production From Challenging Marine Dinoflagellate Samples: A Case Study on Two Benthic <i>Prorocentrum</i> Species

Two-dimensional gel electrophoresis (2-DE) is a major element of conventional gel-based proteomics, which resolves complex protein mixtures. Protein extraction with the removal of interfering substances from the sample remains the key to producing high-quality 2-DE profiles. Marine dinoflagellates c...

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Bibliographic Details
Main Authors: Thomas Chun-Hung Lee, Kaze King-Yip Lai, Celia Sze-Nga Kwok, Steven Jing-Liang Xu, Fred Wang-Fat Lee
Format: Article
Language:English
Published: MDPI AG 2020-05-01
Series:Journal of Marine Science and Engineering
Subjects:
dinoflagellates
protein preparation
TRIzol reagent
two-dimensional gel electrophoresis
<i>Prorocentrum</i>
Online Access:https://www.mdpi.com/2077-1312/8/5/363

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https://www.mdpi.com/2077-1312/8/5/363

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