Probing contacts of inhibitor locked in transition states in the catalytic triad of DENV2 type serine protease and its mutants by 1H, 19F and 15 N NMR spectroscopy

Probing contacts of inhibitor locked in transition states in the catalytic triad of DENV2 type serine protease and its mutants by 1H, 19F and 15 N NMR spectroscopy

Abstract Background Detailed structural knowledge of enzyme-inhibitor complexes trapped in intermediate state is the key for a fundamental understanding of reaction mechanisms taking place in enzymes and is indispensable as a structure-guided drug design tool. Solution state NMR uniquely allows the...

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Bibliographic Details
Main Authors: Peter Agback, Esmeralda Woestenenk, Tatiana Agback
Format: Article
Language:English
Published: BMC 2020-05-01
Series:BMC Molecular and Cell Biology
Subjects:
Dengue
Serine protease
Catalytic triad
NMR
19F, ligand interaction
Online Access:http://link.springer.com/article/10.1186/s12860-020-00283-0

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http://link.springer.com/article/10.1186/s12860-020-00283-0

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