A protocol for generating induced T cells by reprogramming B cells in vivo
Obtaining T cells by reprogramming is one of the major goals in regenerative medicine. Here, we describe a protocol for generating functional T cells from Hoxb5-expressing pro/pre-B cells in vivo. This protocol includes the construction of Hoxb5 recombinant plasmids, retroviral packaging, isolation...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2018-09-01
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| Series: | Cell Regeneration |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2045976918300014 |