CRISPR/Cas9-based editing of a sensitive transcriptional regulatory element to achieve cell type-specific knockdown of the NEMO scaffold protein.

CRISPR/Cas9-based editing of a sensitive transcriptional regulatory element to achieve cell type-specific knockdown of the NEMO scaffold protein.

The use of alternative promoters for the cell type-specific expression of a given mRNA/protein is a common cell strategy. NEMO is a scaffold protein required for canonical NF-κB signaling. Transcription of the NEMO gene is primarily controlled by two promoters: one (promoter B) drives NEMO transcrip...

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Bibliographic Details
Main Authors: Milad Babaei, Yuekun Liu, Shelly M Wuerzberger-Davis, Ethan Z McCaslin, Christopher J DiRusso, Alan T Yeo, Larisa Kagermazova, Shigeki Miyamoto, Thomas D Gilmore
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2019-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0222588

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https://doi.org/10.1371/journal.pone.0222588

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