Analyzing Single Molecule Localization Microscopy Data Using Cubic Splines

Abstract The resolution of super-resolution microscopy based on single molecule localization is in part determined by the accuracy of the localization algorithm. In most published approaches to date this localization is done by fitting an analytical function that approximates the point spread functi...

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Bibliographic Details
Main Authors: Hazen P. Babcock, Xiaowei Zhuang
Format: Article
Language:English
Published: Nature Publishing Group 2017-04-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-017-00622-w