Validation of near infrared fluorescence (NIRF) probes in vivo with dual laser NIRF endoscope.
PURPOSE:The development of NIRF cathepsin activity probes offered the ability to visualize tumor associated tumor reaction and act as a surrogate marker to delineate the dysplastic lesions. One major type is a NIRF substrate of cathepsins (SBP), which is involved in catalytic way to produce high lev...
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doaj-ceaa00d845834e818adbbe535da246192020-11-24T21:52:15ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-011311e020656810.1371/journal.pone.0206568Validation of near infrared fluorescence (NIRF) probes in vivo with dual laser NIRF endoscope.Manisha ShrivastavElias GounarisMohammad W KhanJeffrey KoStacy H RyuMatthew BogyoAndrew LarsonTerrence A BarretDavid J BentremPURPOSE:The development of NIRF cathepsin activity probes offered the ability to visualize tumor associated tumor reaction and act as a surrogate marker to delineate the dysplastic lesions. One major type is a NIRF substrate of cathepsins (SBP), which is involved in catalytic way to produce high levels of fluorescence emission. The other major type (ABP) reacts with active cathepsins in stoichiometric manner since they bind covalently with their active center. Little is known about the sensitivity and the specificity of the NIRF probes to detect autochthonous developed dysplastic lesions. Dual laser NIRF endoscope provides a good tool to determine the efficiency of various NIRF probes in vivo in the same lesions. EXPERIMENTAL DESIGN:In the current study, we validated both types of NIRF probes by using the dual laser NIRF endoscope to detect lesions colon cancer mouse model (TS4Cre/cAPC +/lox). RESULTS:The dual laser NIRF endoscope is emitting equal power with both lasers. It can detect with the same efficiency in 680 mode, as well as, 750 mode when NIFR probes of the same scaffold in vivo. When SBP and ABP were used, our results showed both probes are efficient enough to detect large polyps but small dysplastic lesions could not efficiently imaged with the ABP. CONCLUSIONS:The dual laser NIRF endoscope is a powerful tool to validate probes. The probes that react catalytically with the active center of cathepsins are more efficient than the ones that react stoichiometrically in detecting small lesions.http://europepmc.org/articles/PMC6214553?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Manisha Shrivastav Elias Gounaris Mohammad W Khan Jeffrey Ko Stacy H Ryu Matthew Bogyo Andrew Larson Terrence A Barret David J Bentrem |
spellingShingle |
Manisha Shrivastav Elias Gounaris Mohammad W Khan Jeffrey Ko Stacy H Ryu Matthew Bogyo Andrew Larson Terrence A Barret David J Bentrem Validation of near infrared fluorescence (NIRF) probes in vivo with dual laser NIRF endoscope. PLoS ONE |
author_facet |
Manisha Shrivastav Elias Gounaris Mohammad W Khan Jeffrey Ko Stacy H Ryu Matthew Bogyo Andrew Larson Terrence A Barret David J Bentrem |
author_sort |
Manisha Shrivastav |
title |
Validation of near infrared fluorescence (NIRF) probes in vivo with dual laser NIRF endoscope. |
title_short |
Validation of near infrared fluorescence (NIRF) probes in vivo with dual laser NIRF endoscope. |
title_full |
Validation of near infrared fluorescence (NIRF) probes in vivo with dual laser NIRF endoscope. |
title_fullStr |
Validation of near infrared fluorescence (NIRF) probes in vivo with dual laser NIRF endoscope. |
title_full_unstemmed |
Validation of near infrared fluorescence (NIRF) probes in vivo with dual laser NIRF endoscope. |
title_sort |
validation of near infrared fluorescence (nirf) probes in vivo with dual laser nirf endoscope. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2018-01-01 |
description |
PURPOSE:The development of NIRF cathepsin activity probes offered the ability to visualize tumor associated tumor reaction and act as a surrogate marker to delineate the dysplastic lesions. One major type is a NIRF substrate of cathepsins (SBP), which is involved in catalytic way to produce high levels of fluorescence emission. The other major type (ABP) reacts with active cathepsins in stoichiometric manner since they bind covalently with their active center. Little is known about the sensitivity and the specificity of the NIRF probes to detect autochthonous developed dysplastic lesions. Dual laser NIRF endoscope provides a good tool to determine the efficiency of various NIRF probes in vivo in the same lesions. EXPERIMENTAL DESIGN:In the current study, we validated both types of NIRF probes by using the dual laser NIRF endoscope to detect lesions colon cancer mouse model (TS4Cre/cAPC +/lox). RESULTS:The dual laser NIRF endoscope is emitting equal power with both lasers. It can detect with the same efficiency in 680 mode, as well as, 750 mode when NIFR probes of the same scaffold in vivo. When SBP and ABP were used, our results showed both probes are efficient enough to detect large polyps but small dysplastic lesions could not efficiently imaged with the ABP. CONCLUSIONS:The dual laser NIRF endoscope is a powerful tool to validate probes. The probes that react catalytically with the active center of cathepsins are more efficient than the ones that react stoichiometrically in detecting small lesions. |
url |
http://europepmc.org/articles/PMC6214553?pdf=render |
work_keys_str_mv |
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