Coxsackievirus B Persistence Modifies the Proteome and the Secretome of Pancreatic Ductal Cells
Summary: The group B Coxsackieviruses (CVB), belonging to the Enterovirus genus, can establish persistent infections in human cells. These persistent infections have been linked to chronic diseases including type 1 diabetes. Still, the outcomes of persistent CVB infections in human pancreas are larg...
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2019-09-01
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doaj-ceca93813a654304b2be929c78ac04322020-11-24T20:42:54ZengElsevieriScience2589-00422019-09-0119340357Coxsackievirus B Persistence Modifies the Proteome and the Secretome of Pancreatic Ductal CellsNiina Lietzén0Karoliina Hirvonen1Anni Honkimaa2Tanja Buchacher3Jutta E. Laiho4Sami Oikarinen5Magdalena A. Mazur6Malin Flodström-Tullberg7Eric Dufour8Amir-Babak Sioofy-Khojine9Heikki Hyöty10Riitta Lahesmaa11Turku Bioscience Centre, University of Turku and Åbo Akademi University, FI-20520 Turku, FinlandTurku Bioscience Centre, University of Turku and Åbo Akademi University, FI-20520 Turku, FinlandFaculty of Medicine and Health Technology, Tampere University, FI-33014 Tampere, FinlandTurku Bioscience Centre, University of Turku and Åbo Akademi University, FI-20520 Turku, FinlandFaculty of Medicine and Health Technology, Tampere University, FI-33014 Tampere, FinlandFaculty of Medicine and Health Technology, Tampere University, FI-33014 Tampere, FinlandCenter for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institutet, Karolinska University Hospital, Stockholm 141 86, SwedenCenter for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institutet, Karolinska University Hospital, Stockholm 141 86, SwedenFaculty of Medicine and Life Sciences, BioMediTech Institute and Tampere University Hospital, FI-33014 Tampere, FinlandFaculty of Medicine and Health Technology, Tampere University, FI-33014 Tampere, FinlandFaculty of Medicine and Health Technology, Tampere University, FI-33014 Tampere, Finland; Fimlab Laboratories, Pirkanmaa Hospital District, FI-33520 Tampere, FinlandTurku Bioscience Centre, University of Turku and Åbo Akademi University, FI-20520 Turku, Finland; Corresponding authorSummary: The group B Coxsackieviruses (CVB), belonging to the Enterovirus genus, can establish persistent infections in human cells. These persistent infections have been linked to chronic diseases including type 1 diabetes. Still, the outcomes of persistent CVB infections in human pancreas are largely unknown. We established persistent CVB infections in a human pancreatic ductal-like cell line PANC-1 using two distinct CVB1 strains and profiled infection-induced changes in cellular protein expression and secretion using mass spectrometry-based proteomics. Persistent infections, showing characteristics of carrier-state persistence, were associated with a broad spectrum of changes, including changes in mitochondrial network morphology and energy metabolism and in the regulated secretory pathway. Interestingly, the expression of antiviral immune response proteins, and also several other proteins, differed clearly between the two persistent infections. Our results provide extensive information about the protein-level changes induced by persistent CVB infection and the potential virus-associated variability in the outcomes of these infections. : Biological Sciences; Microbiology; Virology; Proteomics Subject Areas: Biological Sciences, Microbiology, Virology, Proteomicshttp://www.sciencedirect.com/science/article/pii/S2589004219302676 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Niina Lietzén Karoliina Hirvonen Anni Honkimaa Tanja Buchacher Jutta E. Laiho Sami Oikarinen Magdalena A. Mazur Malin Flodström-Tullberg Eric Dufour Amir-Babak Sioofy-Khojine Heikki Hyöty Riitta Lahesmaa |
spellingShingle |
Niina Lietzén Karoliina Hirvonen Anni Honkimaa Tanja Buchacher Jutta E. Laiho Sami Oikarinen Magdalena A. Mazur Malin Flodström-Tullberg Eric Dufour Amir-Babak Sioofy-Khojine Heikki Hyöty Riitta Lahesmaa Coxsackievirus B Persistence Modifies the Proteome and the Secretome of Pancreatic Ductal Cells iScience |
author_facet |
Niina Lietzén Karoliina Hirvonen Anni Honkimaa Tanja Buchacher Jutta E. Laiho Sami Oikarinen Magdalena A. Mazur Malin Flodström-Tullberg Eric Dufour Amir-Babak Sioofy-Khojine Heikki Hyöty Riitta Lahesmaa |
author_sort |
Niina Lietzén |
title |
Coxsackievirus B Persistence Modifies the Proteome and the Secretome of Pancreatic Ductal Cells |
title_short |
Coxsackievirus B Persistence Modifies the Proteome and the Secretome of Pancreatic Ductal Cells |
title_full |
Coxsackievirus B Persistence Modifies the Proteome and the Secretome of Pancreatic Ductal Cells |
title_fullStr |
Coxsackievirus B Persistence Modifies the Proteome and the Secretome of Pancreatic Ductal Cells |
title_full_unstemmed |
Coxsackievirus B Persistence Modifies the Proteome and the Secretome of Pancreatic Ductal Cells |
title_sort |
coxsackievirus b persistence modifies the proteome and the secretome of pancreatic ductal cells |
publisher |
Elsevier |
series |
iScience |
issn |
2589-0042 |
publishDate |
2019-09-01 |
description |
Summary: The group B Coxsackieviruses (CVB), belonging to the Enterovirus genus, can establish persistent infections in human cells. These persistent infections have been linked to chronic diseases including type 1 diabetes. Still, the outcomes of persistent CVB infections in human pancreas are largely unknown. We established persistent CVB infections in a human pancreatic ductal-like cell line PANC-1 using two distinct CVB1 strains and profiled infection-induced changes in cellular protein expression and secretion using mass spectrometry-based proteomics. Persistent infections, showing characteristics of carrier-state persistence, were associated with a broad spectrum of changes, including changes in mitochondrial network morphology and energy metabolism and in the regulated secretory pathway. Interestingly, the expression of antiviral immune response proteins, and also several other proteins, differed clearly between the two persistent infections. Our results provide extensive information about the protein-level changes induced by persistent CVB infection and the potential virus-associated variability in the outcomes of these infections. : Biological Sciences; Microbiology; Virology; Proteomics Subject Areas: Biological Sciences, Microbiology, Virology, Proteomics |
url |
http://www.sciencedirect.com/science/article/pii/S2589004219302676 |
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