Isolation and culture of mouse alveolar type II cells to study type II to type I cell differentiation

Isolation and culture of mouse alveolar type II cells to study type II to type I cell differentiation

Summary: Alveolar type II cells (AT2) are stem cells of lung epithelium. To study these cells, we developed protocols to isolate primary AT2 and test the effects of potential niche factors in regulating the AT2 progenitor functions. AT2 freshly isolated from mouse lungs are grown in 2D or 3D culture...

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Bibliographic Details
Main Authors: Qian Chen, Yuru Liu
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Cell culture
Cell isolation
Cell-based Assays
Model Organisms
Stem Cells
Cell Differentiation
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720302288

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http://www.sciencedirect.com/science/article/pii/S2666166720302288

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