Downregulation of hippocampal NR2A/2B subunits related to cognitive impairment in a pristane-induced lupus BALB/c mice.
Neuropsychiatric systemic lupus erythematosus (NPSLE) is associated with learning and memory deficit. Murine model of lupus induced by pristane in BALB/c mice is an experimental model that resembles some clinical and immunological SLE pathogenesis. Nevertheless, there is no experimental evidence tha...
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2019-01-01
|
Series: | PLoS ONE |
Online Access: | https://doi.org/10.1371/journal.pone.0217190 |
id |
doaj-cf31d0da7a704295b259b3e65ad65d2b |
---|---|
record_format |
Article |
spelling |
doaj-cf31d0da7a704295b259b3e65ad65d2b2021-03-03T21:08:03ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01149e021719010.1371/journal.pone.0217190Downregulation of hippocampal NR2A/2B subunits related to cognitive impairment in a pristane-induced lupus BALB/c mice.Jonatan Luciano-JaramilloFlavio Sandoval-GarcíaMónica Vázquez-Del MercadoYanet Karina Gutiérrez-MercadoRosa Elena Navarro-HernándezErika Aurora Martínez-GarcíaOscar Pizano-MartínezFernanda Isadora Corona-MerazJacinto Bañuelos-PinedaJorge Fernando Floresvillar-MosquedaBeatriz Teresita Martín-MárquezNeuropsychiatric systemic lupus erythematosus (NPSLE) is associated with learning and memory deficit. Murine model of lupus induced by pristane in BALB/c mice is an experimental model that resembles some clinical and immunological SLE pathogenesis. Nevertheless, there is no experimental evidence that relates this model to cognitive dysfunction associated with NR2A/2B relative expression. To evaluate cognitive impairment related to memory deficits in a murine model of lupus induced by pristane in BALB/c mice related to mRNA relative expression levels of NR2A/2B hippocampal subunits in short and long-term memory task at 7 and 12 weeks after LPS exposition in a behavioral test with the use of Barnes maze. A total of 54 female BALB/c mice 8-12 weeks old were included into 3 groups: 7 and 12 weeks using pristane alone (0.5 mL of pristane) by a single intraperitoneal (i.p.) injection. A control group (single i.p. injection of 0.5 mL NaCl 0.9%) and pristane plus LPS exposure using single i.p. pristane injection and LPS of E. coli O55:B5, in a dose of 3mg/kg diluted in NaCl 0.9% 16 weeks post-pristane administration. To determine cognitive dysfunction, mice were tested in a Barnes maze. Serum anti-Sm antibodies and relative expression of hippocampal NR2A/2B subunits (GAPDH as housekeeping gene) with SYBR green quantitative reverse transcription polymerase chain reaction and 2-ΔΔCT method were determined in the groups. Downregulation of hippocampal NR2A subunit was more evident than NR2B in pristane and pristane+LPS at 7 and 12 weeks of treatment and it is related to learning and memory disturbance assayed by Barnes maze. This is the first report using the murine model of lupus induced by pristane that analyzes the NMDA subunit receptors, finding a downregulation of NR2A subunit related to learning and memory disturbance being more evident when they were exposed to LPS.https://doi.org/10.1371/journal.pone.0217190 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jonatan Luciano-Jaramillo Flavio Sandoval-García Mónica Vázquez-Del Mercado Yanet Karina Gutiérrez-Mercado Rosa Elena Navarro-Hernández Erika Aurora Martínez-García Oscar Pizano-Martínez Fernanda Isadora Corona-Meraz Jacinto Bañuelos-Pineda Jorge Fernando Floresvillar-Mosqueda Beatriz Teresita Martín-Márquez |
spellingShingle |
Jonatan Luciano-Jaramillo Flavio Sandoval-García Mónica Vázquez-Del Mercado Yanet Karina Gutiérrez-Mercado Rosa Elena Navarro-Hernández Erika Aurora Martínez-García Oscar Pizano-Martínez Fernanda Isadora Corona-Meraz Jacinto Bañuelos-Pineda Jorge Fernando Floresvillar-Mosqueda Beatriz Teresita Martín-Márquez Downregulation of hippocampal NR2A/2B subunits related to cognitive impairment in a pristane-induced lupus BALB/c mice. PLoS ONE |
author_facet |
Jonatan Luciano-Jaramillo Flavio Sandoval-García Mónica Vázquez-Del Mercado Yanet Karina Gutiérrez-Mercado Rosa Elena Navarro-Hernández Erika Aurora Martínez-García Oscar Pizano-Martínez Fernanda Isadora Corona-Meraz Jacinto Bañuelos-Pineda Jorge Fernando Floresvillar-Mosqueda Beatriz Teresita Martín-Márquez |
author_sort |
Jonatan Luciano-Jaramillo |
title |
Downregulation of hippocampal NR2A/2B subunits related to cognitive impairment in a pristane-induced lupus BALB/c mice. |
title_short |
Downregulation of hippocampal NR2A/2B subunits related to cognitive impairment in a pristane-induced lupus BALB/c mice. |
title_full |
Downregulation of hippocampal NR2A/2B subunits related to cognitive impairment in a pristane-induced lupus BALB/c mice. |
title_fullStr |
Downregulation of hippocampal NR2A/2B subunits related to cognitive impairment in a pristane-induced lupus BALB/c mice. |
title_full_unstemmed |
Downregulation of hippocampal NR2A/2B subunits related to cognitive impairment in a pristane-induced lupus BALB/c mice. |
title_sort |
downregulation of hippocampal nr2a/2b subunits related to cognitive impairment in a pristane-induced lupus balb/c mice. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2019-01-01 |
description |
Neuropsychiatric systemic lupus erythematosus (NPSLE) is associated with learning and memory deficit. Murine model of lupus induced by pristane in BALB/c mice is an experimental model that resembles some clinical and immunological SLE pathogenesis. Nevertheless, there is no experimental evidence that relates this model to cognitive dysfunction associated with NR2A/2B relative expression. To evaluate cognitive impairment related to memory deficits in a murine model of lupus induced by pristane in BALB/c mice related to mRNA relative expression levels of NR2A/2B hippocampal subunits in short and long-term memory task at 7 and 12 weeks after LPS exposition in a behavioral test with the use of Barnes maze. A total of 54 female BALB/c mice 8-12 weeks old were included into 3 groups: 7 and 12 weeks using pristane alone (0.5 mL of pristane) by a single intraperitoneal (i.p.) injection. A control group (single i.p. injection of 0.5 mL NaCl 0.9%) and pristane plus LPS exposure using single i.p. pristane injection and LPS of E. coli O55:B5, in a dose of 3mg/kg diluted in NaCl 0.9% 16 weeks post-pristane administration. To determine cognitive dysfunction, mice were tested in a Barnes maze. Serum anti-Sm antibodies and relative expression of hippocampal NR2A/2B subunits (GAPDH as housekeeping gene) with SYBR green quantitative reverse transcription polymerase chain reaction and 2-ΔΔCT method were determined in the groups. Downregulation of hippocampal NR2A subunit was more evident than NR2B in pristane and pristane+LPS at 7 and 12 weeks of treatment and it is related to learning and memory disturbance assayed by Barnes maze. This is the first report using the murine model of lupus induced by pristane that analyzes the NMDA subunit receptors, finding a downregulation of NR2A subunit related to learning and memory disturbance being more evident when they were exposed to LPS. |
url |
https://doi.org/10.1371/journal.pone.0217190 |
work_keys_str_mv |
AT jonatanlucianojaramillo downregulationofhippocampalnr2a2bsubunitsrelatedtocognitiveimpairmentinapristaneinducedlupusbalbcmice AT flaviosandovalgarcia downregulationofhippocampalnr2a2bsubunitsrelatedtocognitiveimpairmentinapristaneinducedlupusbalbcmice AT monicavazquezdelmercado downregulationofhippocampalnr2a2bsubunitsrelatedtocognitiveimpairmentinapristaneinducedlupusbalbcmice AT yanetkarinagutierrezmercado downregulationofhippocampalnr2a2bsubunitsrelatedtocognitiveimpairmentinapristaneinducedlupusbalbcmice AT rosaelenanavarrohernandez downregulationofhippocampalnr2a2bsubunitsrelatedtocognitiveimpairmentinapristaneinducedlupusbalbcmice AT erikaauroramartinezgarcia downregulationofhippocampalnr2a2bsubunitsrelatedtocognitiveimpairmentinapristaneinducedlupusbalbcmice AT oscarpizanomartinez downregulationofhippocampalnr2a2bsubunitsrelatedtocognitiveimpairmentinapristaneinducedlupusbalbcmice AT fernandaisadoracoronameraz downregulationofhippocampalnr2a2bsubunitsrelatedtocognitiveimpairmentinapristaneinducedlupusbalbcmice AT jacintobanuelospineda downregulationofhippocampalnr2a2bsubunitsrelatedtocognitiveimpairmentinapristaneinducedlupusbalbcmice AT jorgefernandofloresvillarmosqueda downregulationofhippocampalnr2a2bsubunitsrelatedtocognitiveimpairmentinapristaneinducedlupusbalbcmice AT beatrizteresitamartinmarquez downregulationofhippocampalnr2a2bsubunitsrelatedtocognitiveimpairmentinapristaneinducedlupusbalbcmice |
_version_ |
1714818605138313216 |