3D Quantification of Vascular-Like Structures in z Stack Confocal Images

Summary: Optical slice microscopy is commonly used to characterize the morphometric features of 3D cellular cultures, such as in vitro vascularization. However, the quantitative analysis of those structures is often performed on a single 2D maximum intensity projection image, limiting the accuracy o...

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Bibliographic Details
Main Authors: Ulrich Bonda, Anna Jaeschke, Anthony Lighterness, Jeremy Baldwin, Carsten Werner, Elena M. De-Juan-Pardo, Laura J. Bray
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720301672