Contamination-resistant, rapid emulsion-based isothermal nucleic acid amplification with Mie-scatter inspired light scatter analysis for bacterial identification

Abstract An emulsion loop-mediated isothermal amplification (eLAMP) platform was developed to reduce the impact that contamination has on assay performance. Ongoing LAMP reactions within the emulsion droplets cause a decrease in interfacial tension, causing a decrease in droplet size, which results...

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Bibliographic Details
Main Authors: Alexander S. Day, Tiffany-Heather Ulep, Elizabeth Budiman, Laurel Dieckhaus, Babak Safavinia, Tyler Hertenstein, Jeong-Yeol Yoon
Format: Article
Language:English
Published: Nature Publishing Group 2021-10-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-99200-4