Trypan blue dye enters viable cells incubated with the pore-forming toxin HlyII of Bacillus cereus.

Trypan blue is a dye that has been widely used for selective staining of dead tissues or cells. Here, we show that the pore-forming toxin HlyII of Bacillus cereus allows trypan blue staining of macrophage cells, despite the cells remaining viable and metabolically active. These findings suggest that...

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Bibliographic Details
Main Authors: Seav-Ly Tran, Andrea Puhar, Maud Ngo-Camus, Nalini Ramarao
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3167804?pdf=render