Preparation of genetically engineered murine SINE RNA without endotoxin contamination

RNAs have been elucidated to play the critical role in regulating gene expression and to be expected as effective drugs in the treatment of cancer and age-related diseases. RNAs are extracted by SDS-NaCl centrifugation after transformation of E.coli by expression vectors, which is a method to obtain...

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Bibliographic Details
Main Authors: Xin Liu, Baixue Lv, Lifang Yan, Murad Khan, Ning Ji, Suleman Shah, Zhixue Song, Yufang Zhao, Libo Su, Xiufang Wang, Zhanjun Lv
Format: Article
Language:English
Published: Elsevier 2020-01-01
Series:MethodsX
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016120303228