Changing local recombination patterns in Arabidopsis by CRISPR/Cas mediated chromosome engineering
The heterochromatic knob (hk4S) on Arabidopsis chromosome 4 prevents the recombination between accessions with and without hk4S. Here, via egg-cell specific expression of the Cas9 nuclease, the authors demonstrate targeted reversal of the 1.1 Mb long hk4S-inversion in Col-0 and restore the crossover...
Main Authors: | , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2020-09-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-020-18277-z |