A Modified Protocol for Bisulfite Genomic Sequencing of Difficult Samples
<p>Abstract</p> <p>The bisulfite genomic sequencing protocol is a widely used method for analyzing DNA methylation. It relies on the deamination of unmethylated cytosine residues to uracil; however, its high rates of DNA degradation and incomplete cytosine to uracil conversion ofte...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2009-01-01
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Series: | Biological Procedures Online |
Subjects: | |
Online Access: | http://www.biologicalproceduresonline.com/content/11/1/99 |