Monitoring protein turnover during phosphate starvation-dependent autophagic degradation using a photoconvertible fluorescent protein aggregate in tobacco BY-2 cells

Monitoring protein turnover during phosphate starvation-dependent autophagic degradation using a photoconvertible fluorescent protein aggregate in tobacco BY-2 cells

We have developed a system for quantitative monitoring of autophagic degradation in transformed tobacco BY-2 cells using an aggregate-prone protein comprised of cytochrome b5 (Cyt b5) and a tetrameric red fluorescent protein (RFP). Unfortunately, this system is of limited use for monitoring the kine...

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Bibliographic Details
Main Authors: Maiko eTasaki, Satoru eAsatsuma, Ken eMatsuoka
Format: Article
Language:English
Published: Frontiers Media S.A. 2014-04-01
Series:Frontiers in Plant Science
Subjects:
Autophagy
Phosphate
phosphite
turnover
chase experiment
protein aggregate
Online Access:http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00172/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00172/full

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