NmeCas9 is an intrinsically high-fidelity genome-editing platform

NmeCas9 is an intrinsically high-fidelity genome-editing platform

Abstract Background The development of CRISPR genome editing has transformed biomedical research. Most applications reported thus far rely upon the Cas9 protein from Streptococcus pyogenes SF370 (SpyCas9). With many RNA guides, wildtype SpyCas9 can induce significant levels of unintended mutations a...

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Bibliographic Details
Main Authors: Nadia Amrani, Xin D. Gao, Pengpeng Liu, Alireza Edraki, Aamir Mir, Raed Ibraheim, Ankit Gupta, Kanae E. Sasaki, Tong Wu, Paul D. Donohoue, Alexander H. Settle, Alexandra M. Lied, Kyle McGovern, Chris K. Fuller, Peter Cameron, Thomas G. Fazzio, Lihua Julie Zhu, Scot A. Wolfe, Erik J. Sontheimer
Format: Article
Language:English
Published: BMC 2018-12-01
Series:Genome Biology
Subjects:
Cas9
CRISPR
sgRNA
Protospacer adjacent motif
Off-target
Neisseria meningitidis
Online Access:http://link.springer.com/article/10.1186/s13059-018-1591-1

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http://link.springer.com/article/10.1186/s13059-018-1591-1

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