A virus-packageable CRISPR screen identifies host factors mediating interferon inhibition of HIV

A virus-packageable CRISPR screen identifies host factors mediating interferon inhibition of HIV

Interferon (IFN) inhibits HIV replication by inducing antiviral effectors. To comprehensively identify IFN-induced HIV restriction factors, we assembled a CRISPR sgRNA library of Interferon Stimulated Genes (ISGs) into a modified lentiviral vector that allows for packaging of sgRNA-encoding genomes...

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Bibliographic Details
Main Authors: Molly OhAinle, Louisa Helms, Jolien Vermeire, Ferdinand Roesch, Daryl Humes, Ryan Basom, Jeffrey J Delrow, Julie Overbaugh, Michael Emerman
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2018-12-01
Series:eLife
Subjects:
CRISPR
HIV
interferon-stimulated genes
restriction factor
screen
virus replication
Online Access:https://elifesciences.org/articles/39823

Internet

https://elifesciences.org/articles/39823

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