Optimization, validation and application of an assay for the activity of HMG-CoA reductase in vitro by LC–MS/MS

Optimization, validation and application of an assay for the activity of HMG-CoA reductase in vitro by LC–MS/MS

A stable HMG-CoA reductase (HMGR) reaction in vitro was developed by a sensitive, selective and precise liquid chromatography–tandem mass spectrometry (LC–MS/MS) method. The optimized enzyme reaction condition contained 1.5 μg of HMGR, 20 nM of NADPH with 50 min of reaction time. The method was vali...

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Bibliographic Details
Main Authors: Jing Wang, Ji-Ye Sun, Chun-Jie Sha, Yu-Feng Shao, Yan-Hong Liu, You-Xin Li, Zhen-Wen Duan, Wan-Hui Liu
Format: Article
Language:English
Published: Elsevier 2015-12-01
Series:Journal of Pharmaceutical Analysis
Subjects:
Xuezhikang
LC–MS/MS
HMG-CoA reductase
Mevalonolactone
Quality control
Online Access:http://www.sciencedirect.com/science/article/pii/S2095177915300022

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http://www.sciencedirect.com/science/article/pii/S2095177915300022

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