Identification and Evaluation of Suitable Reference Genes for RT-qPCR Analysis in Hippodamia variegata (Coleoptera: Coccinellidae) Under Different Biotic and Abiotic Conditions

Identification and Evaluation of Suitable Reference Genes for RT-qPCR Analysis in Hippodamia variegata (Coleoptera: Coccinellidae) Under Different Biotic and Abiotic Conditions

Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is an accurate and convenient technique for quantifying expression levels of the target genes. Selection of the appropriate reference gene is of the vital importance for RT-qPCR analysis. Hippodamia variegata is one of the most i...

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Bibliographic Details
Main Authors: Jiaoxin Xie, Tinghui Liu, Adel Khashaveh, Chaoqun Yi, Xiaoxu Liu, Yongjun Zhang
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-05-01
Series:Frontiers in Physiology
Subjects:
RT-qPCR analysis
Hippodamia variegata
reference gene
expression stability
biotic conditions
abiotic conditions
Online Access:https://www.frontiersin.org/articles/10.3389/fphys.2021.669510/full

Internet

https://www.frontiersin.org/articles/10.3389/fphys.2021.669510/full

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