Salivary citrullinated proteins in rheumatoid arthritis and associated periodontal disease

Abstract Periodontal disease (PD) can be an important precipitating factor in the production of citrullinated proteins. Its importance is emphasized, but it is not the only way to produce citrullinated proteins. The aim of the current study was to determine the periodontal conditions and the salivar...

Full description

Bibliographic Details
Main Authors: Ildikó Tar, Éva Csősz, Edit Végh, Karin Lundberg, Nastya Kharlamova, Boglárka Soós, Zoltán Szekanecz, Ildikó Márton
Format: Article
Language:English
Published: Nature Publishing Group 2021-06-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-93008-y
id doaj-d0cb1d8048684778a84d973d065145b9
record_format Article
spelling doaj-d0cb1d8048684778a84d973d065145b92021-07-04T11:29:19ZengNature Publishing GroupScientific Reports2045-23222021-06-011111810.1038/s41598-021-93008-ySalivary citrullinated proteins in rheumatoid arthritis and associated periodontal diseaseIldikó Tar0Éva Csősz1Edit Végh2Karin Lundberg3Nastya Kharlamova4Boglárka Soós5Zoltán Szekanecz6Ildikó Márton7Department of Oral Medicine, Faculty of Dentistry, University of DebrecenCentre for Molecular Medicine, Faculty of Medicine, University of DebrecenDivision of Rheumatology, Department of Medicine, Faculty of Medicine, University of DebrecenDivision of Rheumatology, Department of Medicine Solna, Karolinska InstitutetDivision of Rheumatology, Department of Medicine Solna, Karolinska InstitutetDivision of Rheumatology, Department of Medicine, Faculty of Medicine, University of DebrecenDivision of Rheumatology, Department of Medicine, Faculty of Medicine, University of DebrecenDepartment of Restorative Dentistry and Endodontics, Faculty of Dentistry, University of DebrecenAbstract Periodontal disease (PD) can be an important precipitating factor in the production of citrullinated proteins. Its importance is emphasized, but it is not the only way to produce citrullinated proteins. The aim of the current study was to determine the periodontal conditions and the salivary citrullinated protein content in patients with rheumatoid arthritis (RA) compared to healthy controls. We also wished to correlate citrullinated protein levels in the saliva and serum biomarkers with the periodontal status and temporomandibular joint (TMJ) involvement of patients with RA. Twenty-three patients with RA and 17 healthy controls participated the study. Saliva samples were taken: citrulline content of saliva was measured. Blood test results for patients with RA were collected. TMJ disorders were described. Cariological and periodontal indices were registered. Periodontal conditions and periodontal staging were also registered. Comparison of measured values between groups was performed. Intragroup correlation of patients’ values was counted. The prevalence of TMJ complaints was significantly higher in the RA group (8/23) versus controls (1/17). The patients with RA had worse periodontal condition because more patients with RA had gingivitis with a significantly higher bleeding on probing (BOP) (RA: 22.4 ± 25.0%; controls: 6.36 ± 11.6%; p = 0.018). Gingival index (GI) was also significantly higher in the patients than in controls (RA: 0.68 ± 0.58; controls: 0.19 ± 0.38; p = 0.010). The citrullinated protein (relative) content of saliva did not differ significantly (p = 0.147) between patients with RA (1102.2 ± 530.8) and healthy controls (1873.1 ± 1594.9). In RA, the salivary anti-CCP levels positively correlated with PD staging (R = 0.464, p = 0.039) . Control subjects more commonly had healthy gingiva than RA patients. Moreover, in the control group more individuals had intact and reduced height periodontium than periodontitis compared to the RA group. There was no significant difference in the levels of salivary citrulline between patients with RA and controls, despite the significant differences in their periodontal status. Thus, salivary citrulline levels are not associated with RA disease severity.https://doi.org/10.1038/s41598-021-93008-y
collection DOAJ
language English
format Article
sources DOAJ
author Ildikó Tar
Éva Csősz
Edit Végh
Karin Lundberg
Nastya Kharlamova
Boglárka Soós
Zoltán Szekanecz
Ildikó Márton
spellingShingle Ildikó Tar
Éva Csősz
Edit Végh
Karin Lundberg
Nastya Kharlamova
Boglárka Soós
Zoltán Szekanecz
Ildikó Márton
Salivary citrullinated proteins in rheumatoid arthritis and associated periodontal disease
Scientific Reports
author_facet Ildikó Tar
Éva Csősz
Edit Végh
Karin Lundberg
Nastya Kharlamova
Boglárka Soós
Zoltán Szekanecz
Ildikó Márton
author_sort Ildikó Tar
title Salivary citrullinated proteins in rheumatoid arthritis and associated periodontal disease
title_short Salivary citrullinated proteins in rheumatoid arthritis and associated periodontal disease
title_full Salivary citrullinated proteins in rheumatoid arthritis and associated periodontal disease
title_fullStr Salivary citrullinated proteins in rheumatoid arthritis and associated periodontal disease
title_full_unstemmed Salivary citrullinated proteins in rheumatoid arthritis and associated periodontal disease
title_sort salivary citrullinated proteins in rheumatoid arthritis and associated periodontal disease
publisher Nature Publishing Group
series Scientific Reports
issn 2045-2322
publishDate 2021-06-01
description Abstract Periodontal disease (PD) can be an important precipitating factor in the production of citrullinated proteins. Its importance is emphasized, but it is not the only way to produce citrullinated proteins. The aim of the current study was to determine the periodontal conditions and the salivary citrullinated protein content in patients with rheumatoid arthritis (RA) compared to healthy controls. We also wished to correlate citrullinated protein levels in the saliva and serum biomarkers with the periodontal status and temporomandibular joint (TMJ) involvement of patients with RA. Twenty-three patients with RA and 17 healthy controls participated the study. Saliva samples were taken: citrulline content of saliva was measured. Blood test results for patients with RA were collected. TMJ disorders were described. Cariological and periodontal indices were registered. Periodontal conditions and periodontal staging were also registered. Comparison of measured values between groups was performed. Intragroup correlation of patients’ values was counted. The prevalence of TMJ complaints was significantly higher in the RA group (8/23) versus controls (1/17). The patients with RA had worse periodontal condition because more patients with RA had gingivitis with a significantly higher bleeding on probing (BOP) (RA: 22.4 ± 25.0%; controls: 6.36 ± 11.6%; p = 0.018). Gingival index (GI) was also significantly higher in the patients than in controls (RA: 0.68 ± 0.58; controls: 0.19 ± 0.38; p = 0.010). The citrullinated protein (relative) content of saliva did not differ significantly (p = 0.147) between patients with RA (1102.2 ± 530.8) and healthy controls (1873.1 ± 1594.9). In RA, the salivary anti-CCP levels positively correlated with PD staging (R = 0.464, p = 0.039) . Control subjects more commonly had healthy gingiva than RA patients. Moreover, in the control group more individuals had intact and reduced height periodontium than periodontitis compared to the RA group. There was no significant difference in the levels of salivary citrulline between patients with RA and controls, despite the significant differences in their periodontal status. Thus, salivary citrulline levels are not associated with RA disease severity.
url https://doi.org/10.1038/s41598-021-93008-y
work_keys_str_mv AT ildikotar salivarycitrullinatedproteinsinrheumatoidarthritisandassociatedperiodontaldisease
AT evacsosz salivarycitrullinatedproteinsinrheumatoidarthritisandassociatedperiodontaldisease
AT editvegh salivarycitrullinatedproteinsinrheumatoidarthritisandassociatedperiodontaldisease
AT karinlundberg salivarycitrullinatedproteinsinrheumatoidarthritisandassociatedperiodontaldisease
AT nastyakharlamova salivarycitrullinatedproteinsinrheumatoidarthritisandassociatedperiodontaldisease
AT boglarkasoos salivarycitrullinatedproteinsinrheumatoidarthritisandassociatedperiodontaldisease
AT zoltanszekanecz salivarycitrullinatedproteinsinrheumatoidarthritisandassociatedperiodontaldisease
AT ildikomarton salivarycitrullinatedproteinsinrheumatoidarthritisandassociatedperiodontaldisease
_version_ 1721320349267656704