A laser-mediated photo-manipulative toolbox for generation and real-time monitoring of DNA lesions

Summary: With the advancement of laser-based microscopy tools, it is now possible to explore mechano-kinetic processes occurring inside the cell. Here, we describe the advanced protocol for studying the DNA repair kinetics in real time using the laser to induce the DNA damage. This protocol can be u...

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Bibliographic Details
Main Authors: Bindhu K. Madhavan, Zhe Han, Albert Sickmann, Rainer Pepperkok, Peter P. Nawroth, Varun Kumar
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672100407X