A FRET-based biosensor for the quantification of glucose in culture supernatants of mL scale microbial cultivations

A FRET-based biosensor for the quantification of glucose in culture supernatants of mL scale microbial cultivations

Abstract Background In most microbial cultivations d-glucose is the main carbon and energy source. However, quantification of d-glucose especially in small scale is still challenging. Therefore, we developed a FRET-based glucose biosensor, which can be applied in microbioreactor-based cultivations....

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Bibliographic Details
Main Authors: Julia Otten, Niklas Tenhaef, Roman P. Jansen, Johannes Döbber, Lisa Jungbluth, Stephan Noack, Marco Oldiges, Wolfgang Wiechert, Martina Pohl
Format: Article
Language:English
Published: BMC 2019-08-01
Series:Microbial Cell Factories
Subjects:
Sensor beads
Online glucose measurement
Micro cultivation
Glucose binding protein
mTurquoise2
Venus
Online Access:http://link.springer.com/article/10.1186/s12934-019-1193-y

Internet

http://link.springer.com/article/10.1186/s12934-019-1193-y

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