An HPLC procedure for separating polyphosphoinositides on hydroxylapatite.

We describe a method for separation of several phosphoinositides by high performance liquid chromatography (HPLC) for the purposes of identification, analysis, and possible purification of cell phospholipid extracts. The phosphoinositides were separated on an hydroxylapatite column using a solvent s...

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Bibliographic Details
Main Authors: LE Boyle, LA Sklar, AE Traynor-Kaplan
Format: Article
Language:English
Published: Elsevier 1990-01-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520427701