Studies on the Structure and Properties of Membrane Phospholipase A<sub>1</sub> Inclusion Bodies Formed at Low Growth Temperatures Using GFP Fusion Strategy

The effect of cultivation temperatures (37, 26, and 18 °C) on the conformational quality of <i>Yersinia pseudotuberculosis</i> phospholipase A<sub>1</sub> (PldA) in inclusion bodies (IBs) was studied using green fluorescent protein (GFP) as a folding reporter. GFP was fused t...

Full description

Bibliographic Details
Main Authors: Svetlana I. Bakholdina, Anna M. Stenkova, Evgenia P. Bystritskaya, Evgeniy V. Sidorin, Natalya Yu. Kim, Ekaterina S. Menchinskaya, Tatiana Yu. Gorpenchenko, Dmitry L. Aminin, Nikita A. Shved, Tamara F. Solov’eva
Format: Article
Language:English
Published: MDPI AG 2021-06-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/26/13/3936