The ADAMTS5 Metzincin Regulates Zebrafish Somite Differentiation

The ADAMTS5 Metzincin Regulates Zebrafish Somite Differentiation

The ADAMTS5 metzincin, a secreted zinc-dependent metalloproteinase, modulates the extracellular matrix (ECM) during limb morphogenesis and other developmental processes. Here, the role of ADAMTS5 was investigated by knockdown of zebrafish adamts5 during embryogenesis. This revealed impaired Sonic He...

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Main Authors: Carolyn M. Dancevic, Yann Gibert, Joachim Berger, Adam D. Smith, Clifford Liongue, Nicole Stupka, Alister C. Ward, Daniel R. McCulloch
Format: Article
Language:English
Published: MDPI AG 2018-03-01
Series:International Journal of Molecular Sciences
Subjects:
metalloproteinase
extracellular matrix
ADAMTS
somite
muscle
zebrafish
Online Access:http://www.mdpi.com/1422-0067/19/3/766
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spelling doaj-d1b09dce7eca4d44accfa9a3b2b024882020-11-25T00:46:26ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-03-0119376610.3390/ijms19030766ijms19030766The ADAMTS5 Metzincin Regulates Zebrafish Somite DifferentiationCarolyn M. Dancevic0Yann Gibert1Joachim Berger2Adam D. Smith3Clifford Liongue4Nicole Stupka5Alister C. Ward6Daniel R. McCulloch7School of Medicine, Deakin University, Waurn Ponds, Victoria 3216, AustraliaSchool of Medicine, Deakin University, Waurn Ponds, Victoria 3216, AustraliaAustralian Regenerative Medicine Institute, Monash University, Clayton, Victoria 3800, AustraliaSchool of Medicine, Deakin University, Waurn Ponds, Victoria 3216, AustraliaSchool of Medicine, Deakin University, Waurn Ponds, Victoria 3216, AustraliaSchool of Medicine, Deakin University, Waurn Ponds, Victoria 3216, AustraliaSchool of Medicine, Deakin University, Waurn Ponds, Victoria 3216, AustraliaSchool of Medicine, Deakin University, Waurn Ponds, Victoria 3216, AustraliaThe ADAMTS5 metzincin, a secreted zinc-dependent metalloproteinase, modulates the extracellular matrix (ECM) during limb morphogenesis and other developmental processes. Here, the role of ADAMTS5 was investigated by knockdown of zebrafish adamts5 during embryogenesis. This revealed impaired Sonic Hedgehog (Shh) signaling during somite patterning and early myogenesis. Notably, synergistic regulation of myod expression by ADAMTS5 and Shh during somite differentiation was observed. These roles were not dependent upon the catalytic activity of ADAMTS5. These data identify a non-enzymatic function for ADAMTS5 in regulating an important cell signaling pathway that impacts on muscle development, with implications for musculoskeletal diseases in which ADAMTS5 and Shh have been associated.http://www.mdpi.com/1422-0067/19/3/766metalloproteinaseextracellular matrixADAMTSsomitemusclezebrafish
collection DOAJ
language English
format Article
sources DOAJ
author Carolyn M. Dancevic
Yann Gibert
Joachim Berger
Adam D. Smith
Clifford Liongue
Nicole Stupka
Alister C. Ward
Daniel R. McCulloch
spellingShingle Carolyn M. Dancevic
Yann Gibert
Joachim Berger
Adam D. Smith
Clifford Liongue
Nicole Stupka
Alister C. Ward
Daniel R. McCulloch
The ADAMTS5 Metzincin Regulates Zebrafish Somite Differentiation
International Journal of Molecular Sciences
metalloproteinase
extracellular matrix
ADAMTS
somite
muscle
zebrafish
author_facet Carolyn M. Dancevic
Yann Gibert
Joachim Berger
Adam D. Smith
Clifford Liongue
Nicole Stupka
Alister C. Ward
Daniel R. McCulloch
author_sort Carolyn M. Dancevic
title The ADAMTS5 Metzincin Regulates Zebrafish Somite Differentiation
title_short The ADAMTS5 Metzincin Regulates Zebrafish Somite Differentiation
title_full The ADAMTS5 Metzincin Regulates Zebrafish Somite Differentiation
title_fullStr The ADAMTS5 Metzincin Regulates Zebrafish Somite Differentiation
title_full_unstemmed The ADAMTS5 Metzincin Regulates Zebrafish Somite Differentiation
title_sort adamts5 metzincin regulates zebrafish somite differentiation
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1422-0067
publishDate 2018-03-01
description The ADAMTS5 metzincin, a secreted zinc-dependent metalloproteinase, modulates the extracellular matrix (ECM) during limb morphogenesis and other developmental processes. Here, the role of ADAMTS5 was investigated by knockdown of zebrafish adamts5 during embryogenesis. This revealed impaired Sonic Hedgehog (Shh) signaling during somite patterning and early myogenesis. Notably, synergistic regulation of myod expression by ADAMTS5 and Shh during somite differentiation was observed. These roles were not dependent upon the catalytic activity of ADAMTS5. These data identify a non-enzymatic function for ADAMTS5 in regulating an important cell signaling pathway that impacts on muscle development, with implications for musculoskeletal diseases in which ADAMTS5 and Shh have been associated.
topic metalloproteinase
extracellular matrix
ADAMTS
somite
muscle
zebrafish
url http://www.mdpi.com/1422-0067/19/3/766
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