Biological observations in microbiota analysis are robust to the choice of 16S rRNA gene sequencing processing algorithm: case study on human milk microbiota

Biological observations in microbiota analysis are robust to the choice of 16S rRNA gene sequencing processing algorithm: case study on human milk microbiota

Abstract Background In recent years, the microbiome field has undergone a shift from clustering-based methods of operational taxonomic unit (OTU) designation based on sequence similarity to denoising algorithms that identify exact amplicon sequence variants (ASVs), and methods to identify contaminat...

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Bibliographic Details
Main Authors: Shirin Moossavi, Faisal Atakora, Kelsey Fehr, Ehsan Khafipour
Format: Article
Language:English
Published: BMC 2020-09-01
Series:BMC Microbiology
Subjects:
Qiime1
Qiime2
Decontam
Reproducibility
Microbiome
Milk microbiota
Online Access:http://link.springer.com/article/10.1186/s12866-020-01949-7

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http://link.springer.com/article/10.1186/s12866-020-01949-7

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