Simultaneous Subcutaneous and Intranasal Administration of a CAF01-Adjuvanted Chlamydia Vaccine Elicits Elevated IgA and Protective Th1/Th17 Responses in the Genital Tract
The selection of any specific immunization route is critical when defining future vaccine strategies against a genital infection like Chlamydia trachomatis (C.t.). An optimal Chlamydia vaccine needs to elicit mucosal immunity comprising both neutralizing IgA/IgG antibodies and strong Th1/Th17 respon...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2017-05-01
|
Series: | Frontiers in Immunology |
Subjects: | |
Online Access: | http://journal.frontiersin.org/article/10.3389/fimmu.2017.00569/full |
id |
doaj-d2336517e317468b9ed23d777d25ccf0 |
---|---|
record_format |
Article |
spelling |
doaj-d2336517e317468b9ed23d777d25ccf02020-11-24T22:32:27ZengFrontiers Media S.A.Frontiers in Immunology1664-32242017-05-01810.3389/fimmu.2017.00569222004Simultaneous Subcutaneous and Intranasal Administration of a CAF01-Adjuvanted Chlamydia Vaccine Elicits Elevated IgA and Protective Th1/Th17 Responses in the Genital TractJeanette Erbo Wern0Maria Rathmann Sorensen1Anja Weinreich Olsen2Peter Andersen3Frank Follmann4Department of Infectious Disease Immunology, Chlamydia Vaccine Research, Statens Serum Institute, Copenhagen, DenmarkDepartment of Infectious Disease Immunology, Chlamydia Vaccine Research, Statens Serum Institute, Copenhagen, DenmarkDepartment of Infectious Disease Immunology, Chlamydia Vaccine Research, Statens Serum Institute, Copenhagen, DenmarkDepartment of Infectious Disease Immunology, Chlamydia Vaccine Research, Statens Serum Institute, Copenhagen, DenmarkDepartment of Infectious Disease Immunology, Chlamydia Vaccine Research, Statens Serum Institute, Copenhagen, DenmarkThe selection of any specific immunization route is critical when defining future vaccine strategies against a genital infection like Chlamydia trachomatis (C.t.). An optimal Chlamydia vaccine needs to elicit mucosal immunity comprising both neutralizing IgA/IgG antibodies and strong Th1/Th17 responses. A strategic tool to modulate this immune profile and mucosal localization of vaccine responses is to combine parenteral and mucosal immunizations routes. In this study, we investigate whether this strategy can be adapted into a two-visit strategy by simultaneous subcutaneous (SC) and nasal immunization. Using a subunit vaccine composed of C.t. antigens (Ags) adjuvanted with CAF01, a Th1/Th17 promoting adjuvant, we comparatively evaluated Ag-specific B and T cell responses and efficacy in mice following SC and simultaneous SC and nasal immunization (SIM). We found similar peripheral responses with regard to interferon gamma and IL-17 producing Ag-specific splenocytes and IgG serum levels in both vaccine strategies but in addition, the SIM protocol also led to Ag-specific IgA responses and increased B and CD4+ T cells in the lung parenchyma, and in lower numbers also in the genital tract (GT). Following vaginal infection with C.t., we observed that SIM immunization gave rise to an early IgA response and IgA-secreting plasma cells in the GT in contrast to SC immunization, but we were not able to detect more rapid recruitment of mucosal T cells. Interestingly, although SIM vaccination in general improved mucosal immunity we observed no improved efficacy against genital infection compared to SC, a finding that warrants for further investigation. In conclusion, we demonstrate a novel vaccination strategy that combines systemic and mucosal immunity in a two-visit strategy.http://journal.frontiersin.org/article/10.3389/fimmu.2017.00569/fullmucosal immunizationIgAChlamydiavaccineheterologous immunization routesgenital infection |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jeanette Erbo Wern Maria Rathmann Sorensen Anja Weinreich Olsen Peter Andersen Frank Follmann |
spellingShingle |
Jeanette Erbo Wern Maria Rathmann Sorensen Anja Weinreich Olsen Peter Andersen Frank Follmann Simultaneous Subcutaneous and Intranasal Administration of a CAF01-Adjuvanted Chlamydia Vaccine Elicits Elevated IgA and Protective Th1/Th17 Responses in the Genital Tract Frontiers in Immunology mucosal immunization IgA Chlamydia vaccine heterologous immunization routes genital infection |
author_facet |
Jeanette Erbo Wern Maria Rathmann Sorensen Anja Weinreich Olsen Peter Andersen Frank Follmann |
author_sort |
Jeanette Erbo Wern |
title |
Simultaneous Subcutaneous and Intranasal Administration of a CAF01-Adjuvanted Chlamydia Vaccine Elicits Elevated IgA and Protective Th1/Th17 Responses in the Genital Tract |
title_short |
Simultaneous Subcutaneous and Intranasal Administration of a CAF01-Adjuvanted Chlamydia Vaccine Elicits Elevated IgA and Protective Th1/Th17 Responses in the Genital Tract |
title_full |
Simultaneous Subcutaneous and Intranasal Administration of a CAF01-Adjuvanted Chlamydia Vaccine Elicits Elevated IgA and Protective Th1/Th17 Responses in the Genital Tract |
title_fullStr |
Simultaneous Subcutaneous and Intranasal Administration of a CAF01-Adjuvanted Chlamydia Vaccine Elicits Elevated IgA and Protective Th1/Th17 Responses in the Genital Tract |
title_full_unstemmed |
Simultaneous Subcutaneous and Intranasal Administration of a CAF01-Adjuvanted Chlamydia Vaccine Elicits Elevated IgA and Protective Th1/Th17 Responses in the Genital Tract |
title_sort |
simultaneous subcutaneous and intranasal administration of a caf01-adjuvanted chlamydia vaccine elicits elevated iga and protective th1/th17 responses in the genital tract |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2017-05-01 |
description |
The selection of any specific immunization route is critical when defining future vaccine strategies against a genital infection like Chlamydia trachomatis (C.t.). An optimal Chlamydia vaccine needs to elicit mucosal immunity comprising both neutralizing IgA/IgG antibodies and strong Th1/Th17 responses. A strategic tool to modulate this immune profile and mucosal localization of vaccine responses is to combine parenteral and mucosal immunizations routes. In this study, we investigate whether this strategy can be adapted into a two-visit strategy by simultaneous subcutaneous (SC) and nasal immunization. Using a subunit vaccine composed of C.t. antigens (Ags) adjuvanted with CAF01, a Th1/Th17 promoting adjuvant, we comparatively evaluated Ag-specific B and T cell responses and efficacy in mice following SC and simultaneous SC and nasal immunization (SIM). We found similar peripheral responses with regard to interferon gamma and IL-17 producing Ag-specific splenocytes and IgG serum levels in both vaccine strategies but in addition, the SIM protocol also led to Ag-specific IgA responses and increased B and CD4+ T cells in the lung parenchyma, and in lower numbers also in the genital tract (GT). Following vaginal infection with C.t., we observed that SIM immunization gave rise to an early IgA response and IgA-secreting plasma cells in the GT in contrast to SC immunization, but we were not able to detect more rapid recruitment of mucosal T cells. Interestingly, although SIM vaccination in general improved mucosal immunity we observed no improved efficacy against genital infection compared to SC, a finding that warrants for further investigation. In conclusion, we demonstrate a novel vaccination strategy that combines systemic and mucosal immunity in a two-visit strategy. |
topic |
mucosal immunization IgA Chlamydia vaccine heterologous immunization routes genital infection |
url |
http://journal.frontiersin.org/article/10.3389/fimmu.2017.00569/full |
work_keys_str_mv |
AT jeanetteerbowern simultaneoussubcutaneousandintranasaladministrationofacaf01adjuvantedchlamydiavaccineelicitselevatedigaandprotectiveth1th17responsesinthegenitaltract AT mariarathmannsorensen simultaneoussubcutaneousandintranasaladministrationofacaf01adjuvantedchlamydiavaccineelicitselevatedigaandprotectiveth1th17responsesinthegenitaltract AT anjaweinreicholsen simultaneoussubcutaneousandintranasaladministrationofacaf01adjuvantedchlamydiavaccineelicitselevatedigaandprotectiveth1th17responsesinthegenitaltract AT peterandersen simultaneoussubcutaneousandintranasaladministrationofacaf01adjuvantedchlamydiavaccineelicitselevatedigaandprotectiveth1th17responsesinthegenitaltract AT frankfollmann simultaneoussubcutaneousandintranasaladministrationofacaf01adjuvantedchlamydiavaccineelicitselevatedigaandprotectiveth1th17responsesinthegenitaltract |
_version_ |
1725733843033391104 |