A Robust and Universal Metaproteomics Workflow for Research Studies and Routine Diagnostics Within 24 h Using Phenol Extraction, FASP Digest, and the MetaProteomeAnalyzer

A Robust and Universal Metaproteomics Workflow for Research Studies and Routine Diagnostics Within 24 h Using Phenol Extraction, FASP Digest, and the MetaProteomeAnalyzer

The investigation of microbial proteins by mass spectrometry (metaproteomics) is a key technology for simultaneously assessing the taxonomic composition and the functionality of microbial communities in medical, environmental, and biotechnological applications. We present an improved metaproteomics...

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Bibliographic Details
Main Authors: Robert Heyer, Kay Schallert, Anja Büdel, Roman Zoun, Sebastian Dorl, Alexander Behne, Fabian Kohrs, Sebastian Püttker, Corina Siewert, Thilo Muth, Gunter Saake, Udo Reichl, Dirk Benndorf
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-08-01
Series:Frontiers in Microbiology
Subjects:
bioinformatics
software
sample preparation
environmental proteomics
microbial communities
mass spectrometry
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2019.01883/full

Internet

https://www.frontiersin.org/article/10.3389/fmicb.2019.01883/full

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