Live cell imaging and proteomic profiling of endogenous NEAT1 lncRNA by CRISPR/Cas9-mediated knock-in

Live cell imaging and proteomic profiling of endogenous NEAT1 lncRNA by CRISPR/Cas9-mediated knock-in

Abstract In mammalian cells, long noncoding RNAs (lncRNAs) form complexes with proteins to execute various biological functions such as gene transcription, RNA processing and other signaling activities. However, methods to track endogenous lncRNA dynamics in live cells and screen for lncRNA interact...

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Bibliographic Details
Main Authors: Bohong Chen, Shengcheng Deng, Tianyu Ge, Miaoman Ye, Jianping Yu, Song Lin, Wenbin Ma, Zhou Songyang
Format: Article
Language:English
Published: SpringerOpen 2020-05-01
Series:Protein & Cell
Subjects:
CRISPR/Cas9 genome editing
endogenous lncRNA labeling
MS2-MCP
NEAT1
paraspeckle dynamics
Online Access:https://doi.org/10.1007/s13238-020-00706-w

Internet

https://doi.org/10.1007/s13238-020-00706-w

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