Robust Cell-Free Expression of Sub-Pathological and Pathological Huntingtin Exon-1 for NMR Studies. General Approaches for the Isotopic Labeling of Low-Complexity Proteins

Robust Cell-Free Expression of Sub-Pathological and Pathological Huntingtin Exon-1 for NMR Studies. General Approaches for the Isotopic Labeling of Low-Complexity Proteins

The high-resolution structural study of huntingtin exon-1 (HttEx1) has long been hampered by its intrinsic properties. In addition to being prone to aggregate, HttEx1 contains low-complexity regions (LCRs) and is intrinsically disordered, ruling out several standard structural biology approaches. He...

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Bibliographic Details
Main Authors: Anna Morató, Carlos A. Elena-Real, Matija Popovic, Aurélie Fournet, Karen Zhang, Frédéric Allemand, Nathalie Sibille, Annika Urbanek, Pau Bernadó
Format: Article
Language:English
Published: MDPI AG 2020-10-01
Series:Biomolecules
Subjects:
huntingtin protein
low-complexity regions
nuclear magnetic resonance (NMR)
cell-free protein expression
isotopic labeling
site-specific isotopic labeling
Online Access:https://www.mdpi.com/2218-273X/10/10/1458

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https://www.mdpi.com/2218-273X/10/10/1458

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