A Simple and Cost-Effective DNA Preparation Method Suitable for High-Throughput PCR Quantification of Hepatitis B Virus Genomes

A Simple and Cost-Effective DNA Preparation Method Suitable for High-Throughput PCR Quantification of Hepatitis B Virus Genomes

Hepatitis B virus (HBV) is a para-retrovirus that reverse transcribes its pregenomic RNA into relaxed circular DNA inside viral nucleocapsids. The number of HBV genomes produced in vitro is typically quantified using commercial silica-membrane-based nucleic acid purification kits to isolate total DN...

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Bibliographic Details
Main Authors: Eunji Jo, Jaewon Yang, Alexander Koenig, Seung Kew Yoon, Marc P. Windisch
Format: Article
Language:English
Published: MDPI AG 2020-08-01
Series:Viruses
Subjects:
hepatitis B virus
viral DNA
genome equivalents
intracellular
extracellular
DNA preparation
Online Access:https://www.mdpi.com/1999-4915/12/9/928

Internet

https://www.mdpi.com/1999-4915/12/9/928

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